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. 2019 Feb 22;16(5):639–660. doi: 10.1080/15476286.2019.1574161

Figure 8.

Figure 8.

Identification and validation of 2 putative ncRNAs in L. braziliensis. (a) and (d): Regions of chromosomes 33 and 32, enclosing the putative ncRNAs LbrM2903_33_lncRNA177 and LbrM2903_32_lncRNA243, respectively. (b) and (e): extracted and zoomed areas for the corresponding lncRNAs depicting the number of reads in L. braziliensis procyclic, metacyclic and amastigote stages. (c) and (f): Northern blot of L. braziliensis total RNA using specific antisense oligonucleotides to each putative ncRNA. Arrows show multiple bands with approximate sizes to those predicted for the putative ncRNA. Ama: amastigote; Meta: metacyclic; Pro: procyclic; Gene: annotated genes; ncRNA: noncoding RNAs. Thin colorful regions (in a and b) represent divergence between reads and the reference genome.