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CRISPRi represses transcription initiation. The crRNA containing Cascade complex is programmed by the icrRNA to target the promoter (TATA indicated in red) of a gene of interest. Cascade binds to the promoter region blocking access of the RNA polymerase (orange) and thereby transcription initiation. The strain used for CRISPRi has both the cas3 gene and the cas6 gene deleted to avoid target DNA degradation and to optimise the repression effect.
