Table 2.

Strains, plasmids, and bacteriophages.

Strains	Description	Reference
E. coli TG1 RepA+	supE hsd∆5 thi ∆(kac-proAB) F’ (traD36 proAB+ lacIq lacZ∆M15) with chromosomal copy of pWV01 repA	[30]
L. lactis IL1403	Laboratory strain	[31]
L. lactis 1403S	Spontaneous SmR derivative of IL1403	[6]
L. lactis 1403S-RS3c	1403S + pG6 containing pRS3c	This work
L. lactis 1403S-RS3n	1403S + pG6 containing pRS3n	This work
L. lactis 1403S-RS4c	1403S + pG6 containing pRS4c	This work
L. lactis 1403S-RS4n	1403S + pG6 containing pRS4n	This work
L. lactis 1403S-RS9c	1403S + pG6 containing pRS9c	This work
L. lactis 1403S-RS9n	1403S + pG6 containing pRS9n	This work
L. lactis 1403S-RS21c	1403S + pG6 containing pRS21c	This work
L. lactis 1403S-RS21n	1403S + pG6 containing pRS21n	This work
L. lactis 1403S-RS36c	1403S + pG6 containing pRS36c	This work
L. lactis 1403S-RS36n	1403S + pG6 containing pRS36n	This work
L. lactis 1403S-RS53c	1403S + pG6 containing pRS53c	This work
L. lactis 1403S-RS53n	1403S + pG6 containing pRS53n	This work
Plasmids
pG6	pKLM CRISPR-Cas ∆s2-s9	[6]
pGhost9	EmR, TS ori Temperature sensitive vector	[17]
pKLM	CRISPR-Cas	[6]
pRS3c	pGhost9 + RS3c	This work
pRS3n	pGhost9 + RS3n	This work
pRS4c	pGhost9 + RS4c	This work
pRS4n	pGhost9 + RS4n	This work
pRS9c	pGhost9 + RS9c	This work
pRS9n	pGhost9 + RS9n	This work
pRS21c	pGhost9 + RS21c	This work
pRS21n	pGhost9 + RS21n	This work
pRS36c	pGhost9 + RS36c	This work
pRS36n	pGhost9 + RS36n	This work
pRS53c	pGhost9 + RS53c	This work
pRS53n	pGhost9 + RS53n	This work
Phages
bIL170	Host L. lactis IL1403/1403S	[32]
P008	Host L. lactis IL1403/1403S	[15]
P335	Host L. lactis IL1403/1403S	[33]