Table 3.
Primer Name |
Sequence 5ʹ – 3ʹ |
---|---|
GB-VF1194 | CATGTTCAATACGGACACGTA |
GB-VR | AAGCTTTTCCGCGCTATTGT |
InsFspacer | TTCTGGAATGGGATTCAGGATTGTTGATGTGTTGGTATTTGAG |
InsRspacer | ATCCGTGTCGTTCTGTCCACTACGTGTCCGTATTGAACATG |
orf53A_s1 | GCAGAAGAACAGCTACTATTTAAGCAAGAAAC |
orf53A_s2 | GTTTCTTGCTTAAATAGTAGCTGTTCTTCTG |
orf53B_s1 | GCTGTTGAATGTGGTTTGATTAATCTTGATACAGC |
orf53B_s2 | GCTGTATCAAGATTAATCAAACCACATTCAACAG |
orf36A_s1 | GCCGGGTTAACAAAAACAAAATCTGGTTATTTAAGAG |
orf36A_s2 | GCTCTTAAATAACCAGATTTTGTTTTTGTTAACCCGG |
orf36B_s1 | GTAGCTAAATACGTCGGAGTTACCCAGCCAG |
orf36B_s2 | GCTGGCTGGGTAACTCCGACGTATTTAGCTA |
936.101F | GCGAAGCCAACACCTACAAT |
936.56R | GCTAAATACGTCGGAGTTAC |
936.60F | AGGAGTTTCTGGTTCGTCCC |
936.47R | TAGGCGACACCATAACGGTA |
936.94F | CTAAGCCCTCTAATTCTCCT |
936.36R | CACGCTTGACCGCGATTTAA |
936.34F | GAGCAAAGCCTTAGCGATTG |
936.92R | GCTAGATAGTCATGGCGATA |
SpacerF | Forward RS sequence + AAATACAACCGCTCCTCGATAAAAGGGGACGAGAACCATATGATTCAGGTATTGC Depending on spacer size, the 3ʹ end was shortened to obtain a primer of 90 nt |
SpacerR | Reverse complement RS sequence + GTTCTCGTCCCCTTTTATCGAGGAGCGGTTGTATTTAGAGAACTTTAAAAACGTG Depending on spacer size, the 3ʹ end was shortened to obtain a primer of 90 nt |
VF-pG9 | GTGGACAGAACGACACGGAT |
VR-pG9 | TCCTGAATCCCATTCCAGAA |