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. 2018 Oct 2;16(4):461–468. doi: 10.1080/15476286.2018.1502589

Table 3.

List of primers used in the study.

Primer
Name
Sequence 5ʹ – 3ʹ
GB-VF1194 CATGTTCAATACGGACACGTA
GB-VR AAGCTTTTCCGCGCTATTGT
InsFspacer TTCTGGAATGGGATTCAGGATTGTTGATGTGTTGGTATTTGAG
InsRspacer ATCCGTGTCGTTCTGTCCACTACGTGTCCGTATTGAACATG
orf53A_s1 GCAGAAGAACAGCTACTATTTAAGCAAGAAAC
orf53A_s2 GTTTCTTGCTTAAATAGTAGCTGTTCTTCTG
orf53B_s1 GCTGTTGAATGTGGTTTGATTAATCTTGATACAGC
orf53B_s2 GCTGTATCAAGATTAATCAAACCACATTCAACAG
orf36A_s1 GCCGGGTTAACAAAAACAAAATCTGGTTATTTAAGAG
orf36A_s2 GCTCTTAAATAACCAGATTTTGTTTTTGTTAACCCGG
orf36B_s1 GTAGCTAAATACGTCGGAGTTACCCAGCCAG
orf36B_s2 GCTGGCTGGGTAACTCCGACGTATTTAGCTA
936.101F GCGAAGCCAACACCTACAAT
936.56R GCTAAATACGTCGGAGTTAC
936.60F AGGAGTTTCTGGTTCGTCCC
936.47R TAGGCGACACCATAACGGTA
936.94F CTAAGCCCTCTAATTCTCCT
936.36R CACGCTTGACCGCGATTTAA
936.34F GAGCAAAGCCTTAGCGATTG
936.92R GCTAGATAGTCATGGCGATA
SpacerF Forward RS sequence + AAATACAACCGCTCCTCGATAAAAGGGGACGAGAACCATATGATTCAGGTATTGC
Depending on spacer size, the 3ʹ end was shortened to obtain a primer of 90 nt
SpacerR Reverse complement RS sequence + GTTCTCGTCCCCTTTTATCGAGGAGCGGTTGTATTTAGAGAACTTTAAAAACGTG
Depending on spacer size, the 3ʹ end was shortened to obtain a primer of 90 nt
VF-pG9 GTGGACAGAACGACACGGAT
VR-pG9 TCCTGAATCCCATTCCAGAA