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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Adv Healthc Mater. 2019 Jan 4;8(3):e1801146. doi: 10.1002/adhm.201801146

Figure 5.

Figure 5.

A cardiac fibrosis model was established by treating CFs (P5) in the microdevices with 10 ng/mL TGF-β1 for 24 hours prior to compression stimulation, the first group receiving only mechanical stimulation, labeled M; the second group continuously treated with TGF-β inhibiting drug, Tranilast, and mechanical stimulation, labeled DM; the third group continuously treated with TGF-β1 and mechanical stimulation for 7 days, labeled TM; and the last group without any mechanical compression or TGF-β1 treatment, labeled C. (a) Representative confocal images of immunofluorescence stained α-SMA (green) and collagen-I (Col I, red) after 7 days cyclic compression stimulation with different strains from 0 to 20% (n=4). (b) RT-PCR of relative mRNA expression of α-SMA, Col-I, Fnand MMP-2 ofCFsafter 7 days cyclic compression stimulation of different strain from 0 to 20% (n=4). Data depicts fold-change ± standard deviation. *p<0.05,** p<0.01, *** p<0.001.