Skip to main content
. 2019 May 20;129(6):2514–2526. doi: 10.1172/JCI125044

Figure 2. CLEC2-deficient mice exhibit impaired lymphatic flow.

Figure 2

(A–C) Whole-mount images of pulmonary lymphatic vessels (green) using the lymphatic reporter Prox1-EGFP in 4- to 8-week-old Clec2–/– and control mice. (D–F) Immunohistochemical analysis of lung sections stained for VEGFR3 (green) shows lymphatic vessels of 4- to 8-week-old Clec2–/– and control mice. (G–I) Whole-mount SMA-stained (red) images of pulmonary lymphatic vessels (Prox1-EGFP, green) from 4- to 8-week-old Clec2–/– and control mice. (J and K) Fluorescence microscopic images of mLNs from iClec2-KO and control mice 50 minutes after intratracheal administration of dextran-568 (red). (L) Quantification of dextran-568 uptake into mLNs by MFI (AU). Scale bars: 25 μm. Data are representative of at least 5 mice in each group. All values represent the mean ± SEM. ***P < 0.001, by Student’s t test.