Skip to main content
. 2019 Apr 29;129(6):2293–2304. doi: 10.1172/JCI126428

Figure 3. Vascular endothelial TLR4 expression regulates neutrophil recruitment to injured hearts via Trif signaling.

Figure 3

Intravital 2-photon imaging of neutrophil (green) behavior in (A) control WT, (B) TLR4-deficient, (C) CD14-deficient, and (D) Trif-deficient cardiac grafts. Vessels were labeled red after injection of quantum dots. n = 4 per experimental group. (E) Intravascular rolling velocities of neutrophils, (F) density of neutrophils, and (G) percentage of extravasated neutrophils in the experimental conditions displayed in A–D. Intravital 2-photon imaging of neutrophil (green) behavior in hearts that lacked TLR4 expression on (H) vascular endothelial cells (Tie2-Cre TLR4fl/fl) or (I) cardiomyocytes (Myh6-Cre TLR4fl/fl). (J) Intravascular rolling velocities, (K) density of neutrophils, and (L) percentage of extravasated neutrophils in the experimental conditions displayed in H and I as well as control TLR4-floxed heart grafts. Data in E–G and J–L represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001, by 1-way ANOVA followed by post hoc Tukey’s multiple comparisons test. Scale bars: 30 μm.