Klb ISH signal was found predominantly concentrated in the suprachiasmatic nucleus (SCh, a), reticular thalamus (RTh, b), principal sensory trigeminal nucleus (5N, c), and facial nerve nucleus (7N, d). Expression using this technique showed occasional Klb+ cells in the anterior hypothalamus (AH, e, black arrows), paraventricular hypothalamus (PVN, e, black arrows), nucleus of the solitary tract (NTS, f, black arrows), and motor nucleus (XII, f, black arrows), but negligible expression elsewhere in the hypothalamus or hindbrain. Scale bar = 200 μm.