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Uniplex IHC demonstrated the specificity of three antibodies to detect neurons, astrocytes, and glia in the hippocampus. The anti-NeuN antibody labeled neurons in the granular layer of the dentate gyrus (GrDG, a), whereas anti-GFAP and anti-Iba1 antibodies labeled astrocytes (b) and microglia (c), respectively, in the molecular layer of the dentate gyrus (MoDG). Scale bar = 100 μm.
