Table 1. User input protocol parameters for Ventana Discovery ULTRA or Discovery XT for uniplex or duplex ISH.

This table outlines user-modifiable protocol steps for two different tissue types (liver, brain), as well as two ISH assay (uniplex, duplex). All other steps in these assays are hard-programmed by the Ventana Discovery software and are not modifiable by laboratory staff.

ISH step	Uniplex ISH (liver)	Uniplex ISH (brain)	Duplex ISH (brain)
Baking	32 min / 69°C	32 min / 69°C	32 min / 69°C
Deparaffinization	Selected	Selected	Selected
Cell Conditioning	32 minutes / 97°C	24 minutes / 97°C	24 minutes / 97°C
Protease pretreatment	24 minutes / 37°C	12 minutes / 37°C	12 minutes / 37°C
Probe application	4 minutes / 37°C	4 minutes / 37°C	4 minutes / 37°C
Hybridization	2 hours / 43°C	2 hours / 43°C	2 hours / 43°C
AMP 1 (duplex only)	-	-	32 min / 39°C
AMP 2 (duplex only)	-	-	32 min / 39°C
AMP 5	32 min / 39°C	32 min / 39°C	4 min / 39°C
AMP 8 (duplex only)	-	-	4 min / 39°C