Table 2. ISH probes for mouse, cynomolgus monkey (NHP), and human.
Control and experimental probes used for ISH experiments. All probes are annotated based on NCBI accession number and a defined target region. The “Z pairs” column denotes the size of the hybridization scaffold that builds on a given transcript for fast red staining. All FGFR probes are considered pan-isoform with the exception of Mm-FGFR1, which was specifically designed to identify Mm-FGFR1c and not Mm-FGFR1b.
| Probe | ACD Bio Cat. No. | Accession ID | Target Region | Z pairs | Use/Specificity |
|---|---|---|---|---|---|
| dapB | 312039 | EF191515 | 414–862 | 10 | Negative control (bacterial gene) |
| Mm-PPIB | 313919 | NM_011149.2 | 98–856 | 15 | Positive control (high copy number) |
| Mm-Polr2a | 312479 | NM_009089.2 | 2802–3678 | 20 | Positive control (low copy number) |
| Mm-NPY | 313329 | NM_023456.2 | 28–548 | 12 | Positive control probe (brain) |
| Mm-Pomc | 314089 | NM_008895.4 | 19–995 | 10 | Positive control probe (brain) |
| Mm-FGFR1-O1 | 454949 | NM_001079909.2 | 87–4624 | 20 | Designed for the 1c variant of FGFR1, and against the 1b variant #AF176552 |
| Mm-FGFR2 | 443509 | NM_010207.2 | 2–1677 | 18 | Pan-FGFR2 probe |
| Mm-FGFR3 | 440779 | NM_008010.5 | 511–1615 | 20 | Pan-FGFR3 probe |
| Mm-FGFR4 | 443519 | NM_008011.2 | 25–1953 | 17 | No human cross-reactivity |
| Mm-Klb-O1 | 481219 | NM_031180.2 | 2–1825 | 35 | Designed for maximum signal amplification |
| Hs-PECAM | 316729 | NM_001032378.1 | 915–1827 | 20 | Positive control (NHP and human) |
| Hs-FGFR1 | 310079 | NM_023110 | 811–1832 | 23 | Pan-FGFR1 for NHP and human |
| Hs-FGFR2 | 311179 | NM_000141.4 | 1743–3033 | 25 | Pan-FGFR2 for NHP and human |
| Hs-FGFR3 | 310799 | NM_000142.4 | 536–3245 | 28 | Pan-FGFR3 for NHP and human |
| Hs-FGFR4 | 506279 | NM_002011.4 | 151–1690 | 13 | No mouse cross-reactivity |
| Hs-Klb | 445159 | NM_175737.3 | 729–1680 | 20 | Klb for NHP and human |