Fig. 1. Schematic diagram of the experimental protocol.
(A) Diagram shows the irradiation position of PBM treatment. (B) ATP contents in total protein samples were collected at the different time point in naive control group and PBM treatment group (n=4–5). (C) Animals were randomly divided into three groups. Group I: healthy animals without HI (control); Group II: animals that received HI insults plus sham PBM preconditioning (HI); Group III: animals that received HI insults with PBM preconditioning (PBM+HI). PBM pretreatment was initiated 6 hours before HI insults, and the behavior tests were performed from P26 to P29. Brain collections were conducted at P16 and P29 respectively. Brains collected at P16 were used for immunofluorescence staining, Western blotting, and biochemical analyses (n=5–6). Brains collected at P29 were used for Creyl violet (n=5–6). All data are presented as mean ± SD. *P < 0.05 versus PBM sham group.