Skip to main content
. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Biophotonics. 2019 Feb 14;12(6):e201800359. doi: 10.1002/jbio.201800359

Fig. 3. Effects of PBM preconditioning on the shrinkage volume and neuronal density in the hippocampal CA1 region.

Fig. 3.

(A) Representative images of cresyl violet from control (a), HI group (b) and PBM preconditioning group (c). NeuroTraceTM Fluorescent Nissl Staining was performed to mark neurons. Representative immunofluorescence staining of neurons was shown in (d-f). (B) Quantification analysis was performed by analyzing shrinkage volume and counting the survival neurons. (a) Shrinkage volume was quantized by Image-J software (NIH). Shrinkage volume was expressed as a percent of the total area of the contralateral hemisphere. (b) Numbers of surviving neurons was counted and analyzed in (b). All data are expressed as mean ± SD (n = 5–6). *P < 0.05 versus control group, #P < 0.05 versus HI group. Scale bar = 30 μm.