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. Author manuscript; available in PMC: 2019 Jun 3.
Published in final edited form as: ACS Nano. 2017 Nov 1;11(11):11264–11272. doi: 10.1021/acsnano.7b05631

Figure 4. Single-molecule fluorescence anisotropy and fluorescence lifetimes.

Figure 4

(A) Time-resolved fluorescence anisotropy decays of Cy3-labeled DNA origami. The anisotropy decays of the single labeled constructs, G1, G2 and G3 (orange, purple and green line, respectively) show similar behavior while the decay of the triple-labeled construct (G1G2G3, blue line) shows decreased anisotropy confirming homo-FRET occurrence. (B) Fluorescence lifetimes of the same constructs. The lifetime decay of G1G2G3R (blue) shows a reduction in lifetime compared to that of the single-labeled constructs.