Table 2.
Residual fluorescence anisotropy (r∞) and average fluorescence lifetime (τ) of single- and triple-labeled Cy3 constructs. Δr∞ and Δτ indicate the differences of these values for the multi-dye constructs with respect to the average value of the single-dye constructs. Errors are given as 95% confidence intervals.
| G1XX | XG2X | XXG3 | G1G2G3 | G1G2X | G1XG3 | XG2G3 | |
|---|---|---|---|---|---|---|---|
| r∞ | 0.219 ±0.007 |
0.216 ±0.009 |
0.223 ±0.006 |
0.167 ±0.004 |
0.203 ±0.007 |
0.198 ±0.004 |
0.188 ±0.006 |
| Δ r∞ |
0.052 ±0.006 |
0.014 ±0.009 |
0.023 ±0.006 |
0.032 ±0.008 |
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| τ (ns) | 1.57 ±0.04 |
1.34 ±0.06 |
1.52 ±0.09 |
1.09 ±0.03 |
1.20 ±0.07 |
1.27 ±0.02 |
1.27 ±0.05 |
| Δτ (ns) |
0.40 ±0.05 |
0.27 ±0.08 |
0.27 ±0.05 |
0.16 ±0.07 |
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