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. Author manuscript; available in PMC: 2019 Nov 22.
Published in final edited form as: Nature. 2019 May 22;569(7758):729–733. doi: 10.1038/s41586-019-1233-0

Figure 4. Overexpression of Kdm5b histone demethylase abrogates paternal LAD establishment in the zygote.

Figure 4

a, Experimental design. b, H3K9me3 immunostaining in zygotes expressing wild-type and mutant KDM4D. c, Average Dam-lamin B1 signal at LAD boundaries in H3K9me3-manipulated embryos. Signal in maternal pronucei and paternal pronuclei. d, Average H3K4me3 signal at LAD boundaries. e, H3K4me3 Immunostaining of in zygotes expressing wild-type and mutant Kdm5b mRNA. Scale bars: 10 μm. f, Average Dam-lamin B1 signal at LAD boundaries. g, Dam-lamin B1 profiles on maternal and paternal alleles in non-manipulated controls or zygotes expressing KDM5BWT and KDM5B MUT.