| Step | Denature | Anneal | Extend | |
| 1 | 72 0C for 10 min | |||
| 2 | 94 0C for 1 min | 65 0C for 5 min | 72 0C for 15 min | |
| 3-6 | 94 0C for 1 min | 65 0C for 1 min | 72 0C for 10 min | |
| 7-35* | 94 0C for 1 min | 65 0C for 1 min | 72 0C for 2 min | |
*
33 for the population samples
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