Fig. 4.

RAP does not compete with bLF for binding to DP cells. a The RT-PCR products of β-actin (lanes 1), LRP1 (lanes 2) and intelectin-1 (lane 3) were separated by an agarose gel. Intelectin-1 is a lactoferrin receptor expressed by the small intestine. b DP cells were plated onto 96-well tissue culture plates (1 × 10⁴ cells/well) and incubated with biotin-labeled bLF or biotin-labeled BSA in the presence of the indicated concentrations of RAP (0.1–0.4 μM) for 4 h at 4 °C. The binding of biotin-labeled bLF to DP cells was detected by incubation with HRP-conjugated avidin, which was followed by adding the OPD substrate reagent and measuring the absorbance at 492 nm. Data are presented as the mean values ± SD from three independent experiments. ***p < 0.001