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. 2019 Jun 3;10(6):429. doi: 10.1038/s41419-019-1660-8

Fig. 4. TRIM65 regulated the ubiquitination and degradation of TNRC6A in A549 cells.

Fig. 4

a, b A549 cells were transduced with TRIM65 overexpressing lentivirus (TRIM65OE) or Vector lentivirus in the presence and absence of 10 μM MG132 for 48 h. The mRNA (a) and protein (b) expression of TNRC6A was assessed. c Cell lysates from A549 cells were immunoprecipitated (IP) with TNRC6A antibody (Ab) or TRIM65 Ab or IgG, and then subjected to western blotting analysis with TNRC6A Ab or TRIM65 Ab. The lanes labeled Input indicate cell lysate. d A549 cells transduced with TRIM65OE or Vector were lysed, IP with TNRC6A Ab and then subjected to western blotting analysis with ubiquitin Ab. e, f A549 cells were transduced with TNRC6A overexpressing lentivirus (TNRC6A) or Vector for 48 h. Western blotting (e) and real-time PCR analyses (f) were done. ****P < 0.0001 versus Vector