Table 1.
Gene | Primers |
---|---|
TRIM35 |
Forward: 5′ CCCACCAGGAAATGAGATAG 3′ Reverse: 5′ GAAGTCAGCAGAGACAAGAG 3 |
TRIM68 |
Forward: 5′ CAGAAACTGCCAGACAATCC 3′ Reverse: 5′ AGCCTTATCACCCAGAATCC 3′ |
TRIM47 |
Forward: 5′ CGCAGCTTCTCCGTCTGGTTTC 3′ Reverse: 5′ CCAAGGCACGGTCAGCGTATTC 3′ |
TRIM7 |
Forward: 5′ AGGGTGTCCACATAATTGTTG 3′ Reverse: 5′ ATGCCATGAGGCTCTTTATTG 3′ |
TRIM21 |
Forward: 5′ CCCTTTGCTGGGTATGTG 3′ Reverse: 5′ TGCTCCCTCTCATCCTTC 3′ |
TRIM41 |
Forward: 5′ TTTGGGCAAGGCAACATC 3′ Reverse: 5′ TGGTGCGTGGTTTCAATC 3′ |
TRIM65 |
Forward: 5′ CTCAACCCTGTCACTAAGC 3′ Reverse: 5′ CACCTCCCAGAACAAGAAG 3′ |
ATG3 |
Forward: 5′ TGTTTGGCTATGATGAGCAACG 3′ Reverse: 5′ CACATGGGAGGTGGTGGC 3′ |
ATG5 |
Forward: 5′ GATCACAAGCAACTCTGGATGG 3′ Reverse: 5′ AGCCACAGGACGAAACAGC 3′ |
ATG7 |
Forward: 5′ CCAGTGACGCCAGATTTCC 3′ Reverse: 5′ GGCAGGCACAGATGCTATG 3′ |
ATG12 |
Forward: 5′ AGAGCGAACACGAACCATCC 3′ Reverse: 5′ CCCACGCCTGAGACTTGC 3′ |
ATG16L1 |
Forward: 5′ TGACCTGGAGACGGAGTGC 3′ Reverse: 5′ ACTGGTAGAGGTTCCTTTGCTG 3′ |
Beclin1 |
Forward: 5′ AAACCAGATGCGTTATGCCC 3′ Reverse: 5′ TTTCCGTAAGGAACAAGTCGG 3′ |
ULK1 |
Forward: 5′ CAAGAAGAACCTCGCCAAGTC 3′ Reverse: 5′ GGAAGAGCCTGATGGTGTCC 3′ |
ATG16L1 |
Forward: 5′ TGACCTGGAGACGGAGTGC 3′ Reverse: 5′ ACTGGTAGAGGTTCCTTTGCTG 3′ |
TNRC6A |
Forward: 5′ CTGAGTTTGCCAGTGAAGAG 3′ Reverse: 5′ GCACCATTCCAGTGATTGAG 3′ |
GAPDH |
Forward: 5′ AATCCCATCACCATCTTC 3′ Reverse: 5′ AGGCTGTTGTCATACTTC 3′ |
miR-138-5p |
RT primers: 5′ GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATA CGACCGGCCT 3’ Forward: 5′ GCGAGCTGGTGTTGTGAATC 3′ Reverse: 5′ AGTGCAGGGTCCGAGGTATT 3′ |
RNU6–1 |
Forward: 5′ CTCGCTTCGGCAGCACA 3′ Reverse: 5′ AACGCTTCACGAATTTGCGT 3′ |