Fig. 3.

CCL2 is a key monocyte chemo-attractant cytokine in SHH MB. a Schematic of the experiment and results of cytokine analysis. NeuroD2:SmoA1;Atoh1:GFP tumour GFP tissue was extracted under a fluorescence microscope and either dissociated and FACS-sorted to isolate GFP⁺ cells or homogenised (SmoA1 MB); naive cerebellum was used as a control (CB tissue). Images are representative of three independent experiments. b ELISA analysis of CCL2 cytokine level in cerebellum (CB) and medulloblastoma (MB) tissue, N = 9, Mann–Whitney U test, **P < 0.01. c, d Relative Ccl2 (c) and Ccr2 (d) expression levels in FACS-sorted cells, GFP⁺ are tumour cells, GFP⁻ are cells of the tumour microenvironment. N = 6, Mann–Whitney U test, **P < 0.01. e Microarray data analysis of human patient cohort for CCL2 gene¹³. WNT Wingless, SHH Sonic Hedgehog, N = 140 for WNT, N = 437 for SHH, N = 144 for group 3, and N = 326 for group 4. Box plots represent median and quratiles with whiskers indicating range of values. One-way ANOVA with multiple comparisons, F = 19.17, ****P < 0.0001. Data are represented as mean ± S.E.M. for b–d