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. 2019 Jun 3;9:8228. doi: 10.1038/s41598-019-44635-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transiently increased SOCS3 expression in the ipsilateral but not the contralateral hippocampus. (a) Western blots detecting SOCS3 in synaptosomes isolated from the ipsilateral hippocampus of 2 DPI (n = 4 for both sham and TBI), 7 DPI (n = 6 for both sham and TBI), 1 MPI (n = 5 sham, n = 7 TBI), and 3 MPI (n = 4 sham, n = 4 TBI) animals. (b) Quantitative graph of the Western blot analysis showing SOCS3 normalized to β-tubulin. Western blots (c) and corresponding quantification (d) from synaptosomes isolated from the contralateral hippocampus of 2 DPI (n = 4 for both sham and TBI), 7 DPI (n = 6 for both sham and TBI), 1 MPI (n = 5 sham, n = 7 TBI), and 3 MPI (n = 4 sham, n = 3 TBI) animals. Full length Western blots are presented in Supplementary Figs S5 and S6. Statistical significance was determined by unpaired t-test analysis. *p < 0.05; **p < 0.01.