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. 2019 Jun 3;9:8195. doi: 10.1038/s41598-019-44672-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Total number of indeterminate results by protocol considering all data (strains and inoculated seeds); (B) Total number of indeterminate results by protocol considering only strains (i.e. no inoculated seed data); (C) PD rate mean values and standard deviation by protocol for DS1 dataset; (D) PD rate mean values and standard deviation by protocol for DS2 dataset. (E) ND rate mean values and standard deviation by protocol for DS1 dataset; (F) ND rate mean values and standard deviation by protocol for DS2 dataset. The x – axis in all graphs represents the nine protocols tested in this study, from p1 to p9. Please refer to Table 4 for details of each protocol. Different letters indicate values are significantly different, according to Fisher’s Exact Test, for count data with simulated P-values based on 1e + 05 replicates.