. 2019 Jun 3;9:8195. doi: 10.1038/s41598-019-44672-8

Table 2.

Detailed list of the false positive and false negative results obtained with the panel DNA from target and non-target species in this study.

Protocol number	Cross-reactions*	Indeterminate or false negative result
Protocol number	Cross-reactions*	(F. circinatum strain, originating country)**
p1	F. *marasasianum* (5/6)^a	NRRL26431, Japan (5/6)
	F. *pinninemorale* (5/6)^a	310/061, Spain (1/6)
	F. *sororula* (5/6)^a
	F. temperatum (2/6)^a
p2	F. *begoniae* (6/6)^b	LSVM216, France (1/6)
	F. concentricum (1/6)^b	LSVM1221, Spain (1/6)
	F. culmorum (1/6)^b	FcCa01, Spain (1/6)
	F. *fracticaudum* (3/6)^a	FcCa05, Spain (1/6)
	F. *parvisorum* (3/6)^a	FcCa06, Spain (1/6)
	F. pininemorale (2/6)^a	CSF-13, Spain (1/6)
	F. sororula (2/6)^a	2306 BASA, Chile (1/6)
	F. *subglutinans* (4/6)^b
p3	F. culmorum (1/6)^b	NRRL25708, USA (1/6)
	F. *subglutinans* (3/6)^b	NRRL25333, S. Africa (1/6)
		FcCa06, Spain (1/6)
		CSF-18, Spain (1/6)
p4	F. marasasianum (1/6)^a
	F. proliferatum (1/6)^a
	F. *subglutinans* (6/6)^b
p5	F. proliferatum (1/5)^b	CSF8, Spain (1/5)
	F. *subglutinans* (6/5)^b	CSF10, Spain (1/5)
		CSF11, Spain (1/5)
p6	F. acuminatum (1/5)^a	NRRL25708, USA (1/5)
	F. fracticaudum (1/5)^a	NRRL25331, USA (1/5)
	F. graminearum (1/5)^b	NRRL25333, S. Africa (1/5)
	F. incarnatum (1/5)^b	FcCa02, Spain (1/5)
	F. mangiferae (1/5)^b	FcCa05, Spain (1/5)
	F. *marasasianum* (3/5)^a	FC042V, Spain (1/5)
	F. parviporum (1/5)^a	FC035, Spain (1/5)
	F. pininemorale (1/5)^a	CSF1, Spain (1/5)
	F. reticulatum (1/5)^a	CSF2, Spain (1/5)
	F. sacchari (1/5)^b	CSF3, Spain (1/5)
	F. sororula (1/5)^a	CSF4, Spain (1/5)
	F. sporotrichioides (1/5)^b	CSF7, Spain (1/5)
	F. *subglutinans* (4/5)^b	CSF8, Spain (1/5)
	F. torulosum (1/5)^a	CSF11, Spain (1/5)
	F. thapsinum (1/5)^b	CSF12, Spain (1/5)
	F. tricinctum (2/5)^b	CMW1219, S. Africa (1/5)
	F. verticillioides (1/5)^b
p7	F. *temperatum* (4/4)^c
p8	F. *fracticaudum* (4/6)^a	NRRL26431, Japan (6/6)
	F. incarnatum (1/6)^a	LSVM211, France (1/6)
	F. mangiferae (1/6)^a	LSVM216, France (1/6)
	F. *parvisorum* (4/6)^a
	F. proliferatum (1/6)^a
	F. sacchari (1/6)^a
	F. *sororula* (4/6)^a
	F. *subglutinans* (6/6)^a
	F. *temperatum* (5/6)^a
	F. verticillioides (1/6)^a
p9	F. begoniae (1/6)^b
	F. concentricum (2/6)^b
	F. culmorum (1/6)^b
	F. *fracticaudum* (5/6)^a
	F. fractiflexum (1/6)^b
	F. marasasianum (1/6)^a
	F. proliferatum (1/6)^b
	F. sororula (1/6)^a
	F. *subglutinans* (5/6)^b
	F. torulosum (1/6)^a

*Number of participants for which a cross reaction was observed/number of participants involved. Species names in bold indicate a frequent cross-reaction was observed for the protocol with the 0.5 ng µL⁻¹ DNA extract used.

**Number of participants for which the 0.5 ng µL⁻¹ DNA extract of the F. circinatum strain was not picked up by the protocol or yielded an indeterminate result/number of participants involved.

^aNo reference sequence is available for this marker on GenBank for comparison with amplicon sequence, at the time of this study.

^bComparison of the amplicon sequence with IGS F. circinatum reference sequences available on GenBank shows several polymorphisms in the region between primers.

^cComparison of the amplicon sequence with TEF 1alpha F. circinatum reference sequences available on GenBank shows no polymorphism in the region between primers.