Fig. 2.
CLEC5A and TLR2 mediate DV-activated and DV-activated platelet-induced NET formation. a Human neutrophils were pre-treated with isotype control (300 μg/ml) or anti-CLEC5A mAb, followed by incubation with platelets and DV for 3 h at 37 °C. b Alternatively, neutrophils were pre-incubated with anti-CLEC5A mAb and anti-TLR2 mAb, followed by incubation with DV-activated platelets (MOI = 5) or aggretin-activated platelets at 37 °C for 3 h. c Neutrophils from WT (clec5a+/+tlr2+/+), clec5a−/−tlr2+/+, clec5a+/+tlr2−/−, and clec5a−/−tlr2−/− mice were incubated with WT platelets in the presence of DV (NGC-N) for 3 h at 37 °C. NETs were measured by immunofluorescence staining (μm2). Data are mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test). Source data are provided as Source Data file