Fig. 7.

DV induces inflammatory response and lethality via CLEC5A and TLR2. a and b Human macrophages were incubated with DV or DV-EVs (including both MVs and EXOs) for 2 h at 37 °C, washed and then incubated for a further 24 h (a). Alternatively, cells were preincubated with anti-CLEC5A mAb and anti-TLR2 mAb for 1 h before addition of DVs (b). c Spleens of stat1^−/− and stat1^−/−clec5a^−/− mice were collected at day 5 post DV-infection (as described in Fig. 6) and levels of TNF-α, IL-1β, and IL-6 mRNA were determined by RT-qPCR (n = 3 per group). d Stat1^−/− mice (left panels, n = 9 in saline and isotype control group; n = 7 in anti-TLR2 mAb group) and stat1^−/−clec5a^−/− mice (right panels, n = 8 in saline; n = 7 in isotype control group and anti-TLR2 mAb group) were challenged with a lethal dose of DV via intraperitoneal route, and isotype control mAb (100 μg) or anti-TLR2 mAb (100 μg) were injected intraperitoneally at day 0, 2, 4, and 6 post-infection. Survival rate was measured daily up to day 21 post-infection. Data are mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test). Source data are provided as Source Data file