Fig. 1.

Characterization of human MAPT KI mice. a Tau isoforms in MAPT KI mouse brains were determined. Brain extracts derived from 3-month-old WT, heterozygous (hetero) and homozygous (homo) mice were subjected to western blot analysis after dephosphorylation (n = 3 each). b, c The relative expression of 3R and 4R tau were calculated by semi-quantiative RT-PCR. Data reperesent mean ± SEM (n = 6, Stutent t test (t(10) = 1.883, *p = 0.0446). d Amyloid pathology and neuroinflammation were detected in 24-month-old WT, single MAPT KI, App^NL-G-F single-KI and App^NL-G-F /MAPT double-KI mice. The brain sections were triple-immunostained using 82E1 (blue), anti-GFAP (green) and anti-Iba1 antibody (red). Scale bar: 100 µm. e The brain sections of 24-month-old WT, MAPT single-KI, App^NL-G-F single-KI and App^NL-G-F /MAPT double-KI mice were immunostained by cleaved-caspase 3 antibody. Scale bar: 100 µm. Source data are provided as a Source Data file