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. 2019 Jun 3;9:8251. doi: 10.1038/s41598-019-44698-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of Hes1 expression by neuronal stimulation. (a) Immunofluorescence staining for Hes1 (left panels), Arc (middle panels), and c-Fos (right panels) in the primary visual cortex of wild-type mice before (upper panels) and 2 hours after (lower panels) visual stimulation. (b) Double immunofluorescence staining for GFP (left panels), NeuN (middle panels), and merged images (right panels) in the primary sensory cortex of Venus-Hes1 knock-in mice before (upper panels) and 2 hours after (middle panels) electroconvulsive stimulation. Wild-type (WT) mice were shown as a negative control of immunostaining for GFP (lower panels). Cortical layers I-VI are labeled on the right. Scale bars = 100 μm.