a Amino acid sequence alignment of positive phages by Clustal Omega. Different colors represent the physiochemical properties of amino acids. Red colors present small and hydrophobic, Blue presents Acidic, Magenta indicates Basic, Green shows Hydroxyl, sulfhydryl and amine residues. Grey colors indicate unusual amino acids. An asterisk (*) presents conserved residues in scFv. b Structural configuration of selected positive scFvs by protein modeling software Phyre2. Models show linkers connection with VH and VL regions of (i) B30, (ii) C36 and (iii) B129 scFv sequences