Table 4.
The fold changes in gene expression levels in cell lines exposed to H. scoparia extracts using RT2 Profiler PCR Array System
| Gene symbol/alias | Gene name | HeLa cells | CaCo-2 cells | MCF-7 cells | ||||
|---|---|---|---|---|---|---|---|---|
| Hex | Chl | Met | Hex | Met | Chl | Met | ||
| TNF receptor | ||||||||
| TNF/TNF-α | Tumor necrosis factors | 3.45 | − 2.8 | 2.01 | − 1.6 | − 3.6 | 11.2 | 9.19 |
|
FAS/ TNFRSF6 |
Tumor necrosis factor receptor superfamily member 6 precursor | 4.5 | − 3.7 | − 3.2 | 5.09 | − 1.1 | − 3.2 | − 1.2 |
| FASLG | Fas ligand | 3.34 | − 8.3 | 4.57 | – | 2.54 | – | 2.03 |
| FADD | Fas-associated via death domain | 2.32 | − 4.6 | − 2.3 | − 1.3 | − 4.4 | − 3.3 | − 2.3 |
| CRADD | Death domain-containing protein | − 2.2 | 4.57 | 1.6 | − 1.9 | − 1.2 | 2.42 | 4.2 |
| NGFR | Tumor necrosis factor receptor superfamily member 16 | 2.01 | 3.59 | 2.59 | – | − 3.2 | 4.07 | − 1.2 |
| TNFRSF10A | Tumor necrosis factor receptor superfamily. member 10A | 2.8 | − 3.9 | − 5.4 | − 1.4 | 1.2 | 1.25 | − 1.5 |
| TNFRSF10C | Tumor necrosis factor receptor superfamily. member 10C | 5.2 | – | – | − 3.2 | − 2.3 | – | − 1.3 |
| TNFRSF10D | Tumor necrosis factor receptor superfamily. member 10D | 3.12 | − 4.3 | 2.87 | − 1.4 | 2.54 | – | − 1.3 |
| TNFRSF1A | Tumor necrosis factor receptor superfamily. member 1A | 6.8 | 3.9 | 2.06 | – | – | 3.42 | – |
| TNFRSF11B | Tumor necrosis factor receptor superfamily member 11B | – | – | – | –1.78 | –1.40 | –1.02 | –2.54 |
| TNFSF8 | TNF superfamily member 8 | – | – | – | – | − 3.1 | 1.25 | 1.28 |
| TNFSF10 | TNF superfamily member 10 | – | – | – | 11.9 | 1.21 | − 1.4 | 4.45 |
| TNFSF11 | TNF superfamily member 11 | – | – | – | − 1.4 | – | 2.41 | − 1.6 |
| TRAF family | ||||||||
| TRAF1 | TNF receptor-associated factor1 | – | – | 1.23 | 5.68 | 10.1 | − 1.3 | − 3.7 |
| TRAF2 | TNF receptor-associated factor2 | 2.03 | 7.2 | 4.19 | 1.42 | – | − 1.1 | − 1.4 |
| TRAF3 | TNF receptor-associated factor3 | − 5.2 | 4.1 | 2.58 | − 1.3 | – | − 2.9 | − 1.7 |
| TRAF5 | TNF receptor-associated factor5 | – | – | – | − 1.5 | − 1.7 | − 1.3 | – |
| TRAF6 | TNF receptor-associated factor6 | – | – | – | − 1.2 | − 5.4 | − 1.2 | 2.47 |
| BCL2 family | ||||||||
| Anti-apoptotic members | ||||||||
| BCL2 | B cell CLL/lymphoma 2 | − 5.2 | 5.27 | − 7.1 | – | − 2.2 | − 3.6 | − 1.1 |
| BCL2L1 | B-cell lymphoma like X (BCLX) | 2.38 | – | − 3.2 | − 1.4 | − 4.2 | − 2.1 | – |
| MCL1 | Myeloid cell leukemia sequence 1 (BCL2-related) | − 2.4 | 3.78 | − 5.3 | 2.69 | – | − 1.1 | 2.00 |
| BCL2L10 | Bcl-2-like protein 10 | – | – | – | – | – | 1.28 | 2.34 |
| BCL2L2 | Bcl-2-like protein 2 | – | – | – | – | – | 18.5 | 1.90 |
| Pro-apoptotic members | ||||||||
| BH multidomain members | ||||||||
| BAX | Bcl-2-associated X protein | – | – | – | – | − 1.5 | 2.15 | − 1.5 |
| BAK1 | BCL2-antagonist killer 1 | 5.2 | − 7.3 | 6.78 | − 1.3 | − 2.6 | − 2.5 | 1.93 |
| BOK | BCL2-related ovarian killer | 3.5 | − 3.2 | 2.4 | – | − 1.7 | 5.28 | 3.31 |
| BH3-only members | ||||||||
| BBC3 | BCL2 binding component 3 | 4.84 | − 2.7 | 3.4 | – | – | 7.90 | 1.20 |
| BAD | Bcl2 antagonist Of Cell Death | 5.3 | − 4.6 | 4.42 | – | − 1.3 | 1.58 | − 1.5 |
| BID | BH3 interacting domain death agonist | 2.87 | − 4.2 | − 2.3 | − 1.4 | – | − 2.2 | 3.94 |
| BIK | BCL2-interacting killer (apoptosis-inducing) | 4.12 | − 2.3 | − 3.4 | – | – | 2.34 | 3.58 |
| NF-kB family | ||||||||
| NF-kB 1 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 | – | – | – | − 1.3 | − 1.2 | − 2.9 | − 3.1 |
| NF-kB 2 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 | 2.1 | 4.6 | − 2.2 | − 2.5 | − 1.7 | − 1.4 | 2.31 |
| REL | v-rel reticuloendotheliosis viral oncogene homolog (avian) | − 2.3 | − 2.6 | 2.12 | − 1.9 | − 1.2 | 3.98 | − 2.4 |
| RELA | v-rel reticuloendotheliosis viral oncogene homolog B | – | – | – | – | − 1.2 | − 3.5 | − 3.4 |
| RELB | v-rel reticuloendotheliosis viral oncogene homolog B | 2.54 | 3.16 | 2.01 | 4.07 | – | 2.96 | 2.00 |
| Caspases and regulators | ||||||||
| CASP2 | Caspase 2. apoptosis-related cysteine peptidase | – | 2.45 | 3.56 | − 1.3 | 3.21 | − 1.5 | 9.25 |
| CASP3 | Caspase 3. apoptosis-related cysteine peptidase | 4.23 | 7.34 | 6.8 | − 3.2 | − 1.9 | − 4.2 | − 2.4 |
| CASP4 | Caspase 4. apoptosis-related cysteine peptidase | – | – | – | – | – | – | 2.3 |
| CASP5 | Caspase 5. apoptosis-related cysteine peptidase | – | – | – | 2.40 | – | – | 2.02 |
| CASP6 | Caspase 6. apoptosis-related cysteine peptidase | 3.87 | − 2.4 | 2.68 | 3.58 | 4.58 | 9.65 | 8.41 |
| CASP7 | Caspase 7. apoptosis-related cysteine peptidase | − 4.21 | 2.98 | − 3.59 | 1.09 | − 2.1 | 5.46 | − 2.1 |
| CASP8 | Caspase 8. apoptosis-related cysteine peptidase | 8.19 | 2.54 | 4.93 | − 2.4 | − 1.2 | − 2.3 | − 3.6 |
| CASP9 | Caspase 9. apoptosis-related cysteine peptidase | 5.62 | 3.65 | 2.78 | 1.86 | − 2.6 | − 3.4 | − 2.4 |
| CASP1 | Caspase 1. apoptosis-related cysteine peptidase | – | – | 1.50 | − 1.4 | − 1.6 | 1.45 | − 3.2 |
| CASP10 | Caspase 10. apoptosis-related cysteine peptidase | 4.52 | 2.04 | 5.21 | − 4.2 | 6.25 | − 1.5 | – |
| CASP14 | Caspase 14. apoptosis-related cysteine peptidase | – | – | – | – | – | − 1.9 | 2.00 |
| IAP family | ||||||||
| BIRC2 | Baculoviral IAP repeat-containing protein 2 (Inhibitor of apoptosis protein 2) | − 4.58 | − 2.2 | – | − 1.9 | − 1.4 | 3.12 | 2.83 |
| BIRC3 | Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 3) | − 3.05 | 2.04 | − 3.5 | − 1.5 | – | 2.42 | 4.3 |
| BIRC5 | Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor survivin) | 3.98 | 2. 2 | 2. 1 | – | – | − 1.9 | − 1.5 |
| Other proteins | ||||||||
| APAF1 | Apoptotic protease-activating factor 1 (Apaf-1) | 6.05 | 3.12 | 3.25 | 11.8 | 2.03 | 2.10 | 2.45 |
| HTRA2 | Serine protease HTRA2. mitochondrial precursor | 2.86 | 4.71 | 2.34 | − 2.1 | − 1.4 | − 2 | − 3.1 |
| endoG | Endonuclease G. mitochondrial precursor | 3.48 | 2.02 | 2.36 | − 1.59 | – | 5.46 | − 2.00 |
| DFFA | DNA fragmentation factor subunit alpha | − 5.6 | − 2.4 | − 2.5 | 5.46 | – | 4.4 | − 1.2 |
| CAD | CAD protein | 4.36 | 5.9 | 3.63 | − 2.3 | − 3.4 | 4.78 | 6.09 |
| HMGB1 | High mobility group box 1 | 6.65 | 5.2 | 4.7 | − 1.8 | − 3.2 | 2.50 | 2.14 |
| PTEN | Phosphatase and tensin homolog | 2.13 | 2.45 | − 3.1 | 1.54 | 2.14 | 4.10 | 2.49 |
| TP53 | Cellular tumor antigen P53(Tumor suppressor P53) | 12.4 | 8.05 | 7.2 | 3.88 | 2.04 | 2.98 | 2.40 |
| AKT1 | AKT serine/threonine kinase 1 | 2.4 | – | 2.8 | − 3.1 | − 2.7 | − 4.3 | – |
(Hex: n-Hexane, Chl: Chloroform, Met: Methanol)
– No activity