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. 2019 May 28;13:216. doi: 10.3389/fncel.2019.00216

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Copyright © 2019 Bonafé, dos Santos, Ziegler, Umezawa, Ribeiro, Rocha and Ortega.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glioblastoma stem cells (GSCs) are sensitized to dipotassium glycyrrhizinate (DPG). Sphere-forming ability of GSCs in U87MG (A) and T98G (B) cell lines after 24 and 48 h after DPG treatment. Non-treated cell lines were used as controls. Cells were plated in 2% poly (2-hydroxyethyl methacrylate)-coated cell culture flasks in serum-free medium. (C) The graphic shows the average and standard deviation of three independent experiments. Statistics were performed in a two-tailed t-test with P ≤ 0.05. Differences between treated and non-treated cells were significantly observed in U87MG (P = 0.04) and T98G (P = 1E^-04) cell lines.