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. 2019 May 28;10:1182. doi: 10.3389/fimmu.2019.01182

Figure 6.

Figure 6

SFTSV antagonizes IFN-STAT1 signaling via sequestration of STAT1 into NSs IBs and down-regulation of STAT1 abundance. (A–C) HEK293 cells infected with SFTSV were left untreated (A) or treated with IFN-γ (B) or IFN-α (C) for 30 min at 48 hpi and fixed for visualizing the expression and localization of NSs (green) and endogenous STAT1 (red) by IFA. Nuclei were stained with Hoechst. Asterisks indicate the deprivation of STAT1 nuclear accumulation in SFTSV-infected cells. Arrows in the enlarged panels show the sequestration of STAT1 into SFTSV NSs IBs. The intensity line graphs show the green and red signal intensity along the white lines in enlarged merged images. Over-exposed images of the green/NSs channel were also presented to visualize all the infected cells. (D) Cells with or without NSs expression from the experiments of (A–C) were respectively scored for STAT1 nuclear translocation. Percentages of cells with noticeable STAT1 nuclear import were shown.