Figure 1.

Abolished β2-integrin/kindlin-3 link leads to impaired actin dynamics and impaired nuclear MRTF-A shuttling. (A) RhoA activity in WT and KI dendritic cells was measured in serum starved cells (0.3% fetal calf serum (FCS)/RPMI for 1 h) and serum-stimulated cells (15% FCS for 10 min). N = 3, one representative result is shown. (B) F-actin content was assessed via measurement of corrected total cell fluorescence (CTCF) of TRITC-phalloidin stained cells. Baseline level of F-actin was acquired from serum starved cells and F-actin fold change was calculated for 15% FCS-stimulated cells (30 min stimulation). 25–100 cells per condition were measured, N = 3. (C) Immunofluorescence images of serum starved and serum-stimulated WT and KI cells stained with TRITC-phalloidin. *p < 0.05.