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. 2019 May 28;10:1138. doi: 10.3389/fimmu.2019.01138

Figure 2.

Figure 2

Abolished β2-integrin mediated adhesion leads to impaired nuclear MRTF-A shuttling. (A) Total percentages of dendritic cells with nuclear MRTF-A and cytoplasmic MRTF-A are shown. N = 4 and 200 cells per condition were analyzed. MRTF-A staining was performed on non-starved, starved and serum stimulated cells and (B) immunofluorescence images of WT and KI cells are shown after serum stimulation. (C) F-actin content as CTCF of WT and kindlin-3−/− 25–100 dendritic cells per condition were measured, N = 2. (D) Total percentages of kindlin-3−/− and WT dendritic cells with nuclear MRTF-A and cytoplasmic MRTF-A are shown. (E) Total percentages of adhesion on iC3b induced nuclear MRTF-A and cytoplasmic MRTF-A are shown. WT and KI dendritic cells were detached, serum starved in suspension, and stimulated with replating on glass coverslips or on iC3b coated coverslips. (F) Total percentages of WT dendritic cells starved on glass compared to KI dendritic cells seeded overnight on fibronectin with nuclear MRTF-A and cytoplasmic MRTF-A. KI dendritic cells were serum starved and stimulated. (A–F) If not otherwise indicated: N = 3 and 200 cells per condition were analyzed. MRTF-A staining was performed on non-starved, starved, and serum stimulated cells.