Table 2.

Prevalence and sensitivity values determined by Schistosoma mansoni DNA detection assays before treatment in high, moderate, and low endemicity areas.

Endemic area (Prevalence)£	Method (Type of DNA detection assay; gene target; biological sample)	Reference	Study population (N°)	New test prevalence % (KK prevalence¶)	Sensitivity % of new test (CI 95%) (Reference test)
High	rt-PCR (ITS2) Feces	Al-Shehri et al. (32)	258* SC	67.4 (44,1)	85 (78.4–89.9) (POC-CCA)
					90.2 (84.2–96.2) (LCA)
	LAMP (121-bp tandem repeat) Feces	Mwangi et al. (33)	383 SC	45 (46)	97.2 (93.5–98.8) (K-K)
	PCR-ELISA (121-bp tandem repeat) Urine	Lodh et al.(34)	100 GP	89 (51)	100 (95–100) (K-K/PCR)
	rt-PCR (121-bp tandem repeat) Feces	Fuss et al. (35)	305 SC	92.9 (85.2)	96.8 (94.9–98.8) (K-K)
					98.7 (96.5–100) (LCA)
Moderate low	PCR-ELISA (121-bp tandem repeat) Feces	Gomes et al. (36)	206 GP	30.1 (13.1)	96.3 (81.7–99.8) (K-K)
	PCR (121-bp tandem repeat) Urine	Enk et al. (7)	194 GP	41.2 (13.9)	100 (94.7–100) (Combined 1**)
Low	SmMIT-LAMP (mitochondrial S. mansoni minisatellite DNA region) Feces	Gandasegui et al. (37)	427* GP	30.2 (3.04)	92.9 (66.1–99.8) (K-K)
	r-t PCR (COX-1) Feces	Cavalcanti et al. (30)	108 GP	29.6 (7.4)	100 (63.1–100) (K-K)
	PCR (121-bp tandem repeat) Urine	Hessler et al. (38)	111 SC	78 (8.0)	100 (96–100) (K-K)

^£Classified according WHO; rt-PCR, Real-Time Polymerase Chain Reaction; LAMP, Loop-Mediated Isothermal Amplification Assay; SmMIT-LAMP, Sm Mitochondrial Loop-Mediated Isothermal Amplification Assay; SC, School Children; GP, General Population; KK, Kato-Katz test; POC-CCA, Point-of-Care platform for detection of cathodic circulating antigen; LCA, Latent Class Analysis;

^¶KK duplicate (Two slides/sample);

^*Number of tested samples;

^**Combined 1(parasitological tests): KK, Saline Gradient and the Miracidia Hatch test served as combined reference test.