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. 2019 May 28;10:504. doi: 10.3389/fgene.2019.00504

Figure 3.

Figure 3

(A) Analysis of mouse Slc10a7 transcripts distribution by in situ hybridization. Sections illustrate Slc10a7 expression in the developing bone, vertebrae, molars, and incisors. Developmental stages and section planes are: E14.5 frontal (a,b), E16.5 sagittal (c–h); E18.5 sagittal (i–l) sections. Am, ameloblasts; DP, dental papila; EL, epithelial loop; GUB, gubernaculum; IDE, inner dental epithelium; LI, lower incisor; Od, odontoblasts; ODE, outer dental epithelium; SR, stellate reticulum; Ve, vertebrae. Scale bars: 10 μm (b); 40 μm (a,c,g,h); 30 μm (d,l); 60 μm (i,j); 80 μm (e,f); 150 μm (k). (B) SLC10A7 expression in control and patient skin fibroblasts as detected by western blot. The stain-free membrane, displaying the total amount of protein loaded, was used for quantification. The western blot shown corresponds to one significant experiment (n = 3). The data plotted were the mean of three independent experiments. Statistical analysis was done with the t-test and the p was determined *p < 0.05. (C) Calcium localization in control and patient fibroblasts. The cells were stained for 15 min or 30 min with 4 μm of Fluo4, then washed for 15 or 30 min, respectively and observed by fluorescent microscopy. Images were taken with a 400× magnification, scale bar 10 μm.