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. 2019 May 28;10:579. doi: 10.3389/fphar.2019.00579

FIGURE 6.

FIGURE 6

Increased IL1RA expression in PB-MSCs by TNFα was involved in NF-κB signaling pathway. (A) TNFα (50 ng/mL) increased the levels of NF-κB p-p65 and p65 in both BM-MSCs and PB-MSCs as detected by Western blot, especially in PB-MSCs. (B) Semi-quantitative analysis of results in A showed a higher ratio of NF-κB p-p65/p65 in PB-MSCs treated with TNFα (50 ng/ml) compared to BM-MSCs. n = 5, &p < 0.05, vs. PB-MSCs; #p < 0.05, vs. PB-MSCs; ∗p < 0.05, vs. BM-MSCs. (C) Typical results of IL1RA expression in PB-MSCs as determined by flow cytometry. (D) Semi-quantitative analysis of results in C showed that TNFα (50 ng/mL) induced the expression of IL1RA in PB-MSCs, which could be obviously abolished by NF-κB inhibitor PDTC (100 μM). n = 5, &p < 0.05, vs. PB-MSCs treated without TNFα and PDTC;#p < 0.05, vs. PB-MSCs treated without TNFα and PDTC; ∗p < 0.05, vs. BM-MSCs treated with TNFα.