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. 2019 May 28;10:579. doi: 10.3389/fphar.2019.00579

FIGURE 7.

FIGURE 7

BM-MSCs treated with G-CSF/AMD3100 showed stronger migration potential. (A) Typical image of BM-MSCs migration treated with G-CSF and/or AMD3100 using the Transwell system. (B) Combined treatment of AMD3100 and G-CSF promoted greater BM-MSCs migration than single treatment of AMD3100 or G-CSF. n = 5, @p < 0.05, vs. BM-MSCs treated with or without G-CSF; #p < 0.05, vs. BM-MSCs treated with G-CSF. (C) Combined treatment of AMD3100 and G-CSF induced IL-1RA expression in BM-MSCs as detected by ELISA. n = 3, @p < 0.05, vs. BM-MSCs treated with or without G-CSF; #p < 0.05, vs. BM-MSCs treated with either G-CSF or AMD3100. (D,E) Combined treatment of AMD3100 and G-CSF increased IL-1RA protein levels in BM-MSCs as analyzed by Western blot (D) and semi-quantitative analysis (E). n = 3, ∗p < 0.05, vs. BM-MSCs treated without either AMD3100 or G-CSF; %p < 0.05, vs. BM-MSCs treated without either AMD3100 or G-CSF; #p < 0.05, vs. BM-MSCs treated with either AMD3100 or G-CSF.