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. 2019 May 28;10:1189. doi: 10.3389/fmicb.2019.01189

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Copyright © 2019 Cai, Wang, Le, Wu, Cheng, Guo, Ji, Xi, Zhao, Wang, Xue, Wang, Zhang, Fu, Sun, Feng, Lei, Yang, ur Rahman, Liu, Han and Gu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity of K. pneumoniae K7(ΔGT-1), K7(ΔGT-2), and K7(ΔwcaJ) to GH-K3. (A) Spot and plaque forming assays. Five μl of GH-K3 solutions were spotted onto freshly seeded lawns of K. pneumoniae K7(ΔGT-1), K7(ΔGT-2), and K7(ΔwcaJ) for 6 h at 37°C before observation (top). Meanwhile, the sensitivity of K. pneumoniae K7(ΔGT-1), K7(ΔGT-2), and K7(ΔwcaJ) to GH-K3 were determined by plaque assays (bottom). K7 was used as a control. (B) Adsorption efficiency. The adsorption efficiency of GH-K3 binding to K. pneumoniae K7(ΔGT-1), K7(ΔGT-2), and K7(ΔwcaJ) were determined. ^∗ and ^∗∗ significant differences at P < 0.05 and P < 0.01, respectively. Data represent the mean ± SEM of triplicate experiments.