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. 2019 May 28;10:548. doi: 10.3389/fphar.2019.00548

FIGURE 2.

FIGURE 2

Fibronectin type III domain-containing protein 5 (FNDC5) is transcriptional target gene of FXR. (A,B) Luciferase assay of transient transfection in HEK293T cells with the FNDC5 natural and mutant promoter reporter (n = 3). (C) HepG2 cells were transfected with or without FXR vector, then treated with CDCA (100 μM) or GW4064 (2.5 μM) for 24 h. ChIP was performed with anti-FXR antibody (n = 3). (D) The relative band intensity of PCR products revealed the recruitment of FXR to FNDC5. Data are mean ± SEM. ∗P < 0.05.