Table 4.

Semiquantitative analysis of diaphragm electron microscopy.

	C		ELA
	SAL	LPS	SAL	LPS
Disorganized I-band	1 (0.50–1)	1 (1–1.75)	4 (3–4) *	3.5 (3–4) †
Thickened Z lines	1 (1–1.5)	1.5 (1–2)	3 (2–3) *	4 (4–4) † , #
Mitochondrial subsarcolemmal aggregates	1 (1–1.5)	2 (1.25–2)	3 (2.5–3) *	4 (4–4) † , #
Mitochondrial cristae distortion	0 (0–1.5)	1.5 (1–2)	3 (3–4) *	4 (3.25–4) † , #
Abnormal mitochondrial forms	0 (0–1)	1.5 (1–2)	2 (2–2.5) *	4 (3.25–4) † , #

Pathologic findings were graded on a five-point, semiquantitative, severity-based scoring system, where 0, normal diaphragm tissue; 1, changes in 1–25% of examined tissue; 2, changes in 26–50% of examined tissue; 3, changes in 51–75% of examined tissue; and 4 = changes in 76–100% of examined tissue. C, control; ELA, animals treated with intratracheal instillations of elastase; SAL, saline; LPS, E. coli lipopolysaccharide. Analyses performed using ANOVA on ranks followed by Dunn’s test. Values are median (interquartile range) of six animals in each group.

^*Significantly different from C-SAL group (p < 0.05).

^†Significantly different from C-LPS group (p < 0.05).

^#Significantly different from ELA-SAL group (p < 0.05).