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. 2019 May 28;10:1208. doi: 10.3389/fmicb.2019.01208

FIGURE 7.

FIGURE 7

RT-qPCR validation of differentially expressed genes involved in primary metabolism, secondary metabolism, and the defense response in shoots of A. lancea after the fungal endophyte Gilmaniella sp. AL12 inoculation. Elongation factor 1 alpha gene (EF1a) was used as an internal reference, and the 2−ΔΔCt method was used to analyze the relative mRNA expression. Values are the means of three independent experiments. Bars represent standard deviations. RCA, ribulose-1,5-bisphosphate carboxylase/oxygenase activase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PDHE, pyruvate dehydrogenase; GS, glutamine synthetase; GOGAT, glutamate synthase; DXS, 1-deoxy-d-xylulose 5-phosphate synthase; FS, β-farnesene synthase; CPS1, β-caryophyllene synthase 1; CPS2, β-caryophyllene synthase 2; PLD, Phospholipase D; FLS2, LRR receptor-like kinase FLS2; RPM1, disease resistance protein RPM1; RPS5, disease resistance protein RPS5; WRKY29, transcription factor WRKY29; PR1, pathogenesis-related protein1.