FIGURE 5.

Generation of reactive astrocytes from oligodendrocyte lineage cells around an implanted microelectrode array. Following microelectrode implantation, astrocytes expressed the oligodendrocyte transcription factor Olig2, which may implicate oligodendrocyte lineage cells as a source of astrocytes following injury. (A) Representative immunohistochemical stains for GFAP (green) and Olig2 (red) at 1, 3, 7, and 28 days post-insertion revealing a distinct subpopulation of Olig2+GFAP+ astrocytes. White arrows denote co-localized cells. Center of the probe hole is denoted by a white “x.” Scale bar = 50 μm. (B) Green- and red-only channels for GFAP and Olig2 staining, respectively, at 28 days post-insertion. White arrows denote GFAP+ (left) or Olig2+ (right) cells. Center of the probe hole is denoted by a white “x.” Scale bar = 50 μm. (C) Percent of GFAP+Olig2+ cells over total Olig2+ cells within 50 μm bins up to 300 μm away from site of insertion suggest preference for Olig2+GFAP+ cells to reside near site of probe implantation. ^∗ indicates p < 0.05.