FIGURE 6.

Knockdown of BC032585 lncRNA increases MDR1 expression in MDA-MB-231 breast cancer cells. (A,B) MDA-MB-231 cells were transiently transfected with a specific BC032585 siRNA (100 nM) or a control siRNA for 48 h (A) and 96 h (B). The transfected cells were then harvested for quantification of MDR1 protein levels by Western blot analysis. β-actin was used as an internal loading control. The protein levels were expressed as fold of corresponding controls, and the data are mean ± SD of two or three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01 compared to the corresponding control.