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. 2019 May 28;9:425. doi: 10.3389/fonc.2019.00425

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Copyright © 2019 Goepfert, Dinsart, Rommelaere, Foerster and Moehler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of regulatory T cell (Treg) activity induced by H-1PV infected and uninfected Mz7Mel. Treg* functioned as positive control and are activated with functional anti CD3 and anti CD28. Measurement of proportion of CD4⁺FoxP3⁺GranzymB⁺ cells by FACS analysis (A) and determination of the IL-10 (B) and TGF-ß (C) level in the supernatant (ELISA) with and without ipilimumab and nivolumab. Experiments were performed independently as triplicate *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. ELISA, enzyme linked immunosorbent assay; FACS, fluorescence-activated cell sorting.