Figure 6.

Determination of CTL clone IVSB cytotoxicity induced by H-1PV after 2 days of co-culture with melanoma cells. Measurement of the proportion of CD3⁻CD8⁻CPD⁺PI⁺ cells by FACS analysis. (A) Co-culture with Sk29Mel cells and (B) with Mz7Mel with and without ipilimumab and nivolumab. Experiments were performed independently three times *P ≤ 0.05; **P ≤ 0.01; P ≤ 0.001. CPD, cell proliferation dye eFluor 670; PI, propidium iodide; CTL, human cytotoxic T lymphocytes; FACS, fluorescence-activated cell sorting.