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. 2019 May 28;10:362. doi: 10.3389/fgene.2019.00362

Figure 2.

Figure 2

FGF gradient controls explant migration and ERK1/2 activation. (A) Analysis of LG explant migration towards the FGF3, 7, and 10-soaked beads. Migration was estimated as the change of the distance between bead and the tip of the explant. (B,C) A253 cells were incubated with 1.5 nM FGF3 (A) or FGF10 (B), and phospho-ERK1/2 was monitored by Western blots. Values were normalized to the amount of total protein (total ERK1/2). (C) The graphic representation of the result. n = 3; P < 0.01. FGF10 induces longer MAPK/ERK1/2 activation than FGF3 does. (D–F) Blocking integrin β1 decreases speed of epithelial explant migration toward the FGF3-loaded bead. LG epithelial explants grown near FGF3 beads were treated with Control IgG (D) or the blocking antibody against the integrin subunit β1 (E). (F) Quantification of epithelial growth shown in (D,E). Result represents 3 independent experiments (12 control and 13 treated buds), p < 0.05.