The effect of iron chelation with BPDS on pure bacterial cultures.
A strain of E. coli isolated from human gut (1A), S. Typhimurium (1B), B. thetaiotaomicron (1C) B. longum (1D) and L. rhamnosus (1E) were cultured anaerobically overnight in selective rich media. Cultures were supplemented with BPDS, at 0 (control), 10, 20 and 50 μM. Optical density measurements were taken every 10 min for a period of 24 h and growth curves were plotted for each strain. Data shown = mean and are representative of 3 independent experiments. The data was statistically analysed using, one-way ANOVA, comparing the different concentrations of BPDS to the control, over a period of 24 h; **P<.01, ****P<.0001 versus control (0 μM BPDS).