Table 1.
The effect of iron chelation via BPDS on gut-derived bacterial communities
|
Bacterial Group |
Viable Counts % (“Control” normalised to 100%, ±SEM) |
|||
|---|---|---|---|---|
| T8 |
T24 |
|||
| Control | (+)BPDS | Control | (+)BPDS | |
| Total Anaerobes | 100 | 27.83±9.47 * | 100 | 29.30±7.46 * |
| Bacteroides | 100 | 68.71±31.61 | 100 | 29.70±18.47 |
| Bifidobacteria | 100 | 5.38±1.05 *** | 100 | 110±106.30 |
| Clostridia | 100 | 63.58±25.84 | 100 | 432.89±299.18 |
| Lactobacilli | 100 | 8.21±5.10 ** | 100 | 35.47±5.92 ** |
| Enterobacteriaceae | 100 | 19.01±11.19 * | 100 | 17.65±15.04 * |
Two vessels were set up containing nutritive media and faecal inocula; one vessel was supplemented with BPDS (70 μM BPDS) whilst the other was used as a control (0 μM BPDS). Samples were taken from each vessel at 0, 8, and 24 h, and then enumerated using selective agar plates. Data shown = mean of 3 individual experiments±S.E.M. The data was statistically analysed using unpaired t-test, assuming unequal variance; *P<.05, **P<.01, ***P<.001 and ****P<.0001 for Control versus viable counts at the relevant time-point.