Figure 3.

Effect of sodium arsenite and dimethylarsenic acid on cell apoptosis. MA-10 cells were treated without or with sodium arsenite (10 and 100 µM) and dimethylarsenic acid (1 and 10 mM) for 12 and 24 h. (A) Apoptotic status of the arsenic-treated cells was determined by Annexin V/PI double staining. The proportion of negative (viable) cells, Annexin V-positive (early apoptotic) cells, PI-positive (dead) cells, and Annexin V and PI double-positive (late apoptotic) stained cells are shown following (B) sodium arsenite (10 and 100 µM) and (C) dimethylarsenic acid (1 and 10 mM) treatments. The differences in Annexin V-positive stained cells (early and late apoptotic) following (D) sodium arsenite and (E) dimethylarsenic acid treatments were further analyzed. Data represent the means ± standard error of the mean of three separate experiments. *P<0.05. FITC, fluorescein isothiocyanate; PI, propidium iodide.